PNC-27

PNC-27 is a chimeric peptide combining a p53-derived HDM2-binding domain with a membrane-penetrating leader sequence. Selectively targets surface HDM2 on transformed cells for cancer biology research.

PNC-27 is a rationally designed chimeric peptide consisting of two distinct functional domains: an HDM2-binding sequence derived from the p53 tumor suppressor transactivation domain (residues 12-26) and a hydrophobic membrane-penetrating leader sequence. The design exploits a remarkable biological discovery: while HDM2 (human double minute 2, the E3 ubiquitin ligase that targets p53 for proteasomal degradation) is normally an intracellular protein, it is aberrantly expressed on the outer cell surface membrane of transformed (cancerous) cells but not on normal, untransformed cells. This differential surface expression creates a tumor-specific targeting opportunity.

The mechanism of PNC-27 action involves selective binding to surface-expressed HDM2 on transformed cells, followed by membrane reorganization and pore formation leading to necrotic cell lysis. The p53-derived binding domain provides HDM2 target specificity, while the membrane-penetrating leader sequence drives membrane insertion and disruption upon target engagement. Critically, because normal cells do not express HDM2 on their surface, PNC-27 shows no significant cytotoxicity to untransformed cells in co-culture experiments — demonstrating the selectivity that defines its research value.

Published studies have documented PNC-27’s selective cytotoxicity against multiple transformed cell lines including breast cancer (MDA-MB-231, MCF-7), pancreatic cancer (MIA PaCa-2), leukemia, and melanoma cells, while sparing co-cultured normal fibroblasts, lymphocytes, and epithelial cells. The chimeric peptide’s mechanism is notable for being independent of intracellular p53 status — it functions through surface HDM2 binding rather than intracellular p53-HDM2 interaction. This means it retains activity in p53-null and p53-mutant cancer cell lines, which are resistant to many conventional approaches that rely on p53 pathway restoration.

Supplied as a lyophilized powder with ≥99% purity. Store at -20°C desiccated. For cancer biology and membrane signaling research only.

Product Specifications

Certificate of Analysis

Every batch ships with a Janoshik-verified COA covering HPLC purity, mass spectrometry confirmation, and batch traceability.

Shipping & Lead Times

• US warehouse: ships within 1–2 business days · estimated 3–5 day delivery
• HK warehouse: ships within 2–3 business days · estimated 7–14 day international delivery
• Carriers: DHL Express, FedEx International, UPS Worldwide
• Discreet outer packaging — no peptide branding visible
• Tracking number emailed on dispatch

Lost or Detained Packages

If your shipment is lost or detained by customs, we will reship once free of charge. Contact us with your order number.